https://pubs.rsc.org/en/content/articlehtml/2024/fo/d4fo00991f
Food Funct., First published on 27th April 2024 DOI: 10.1039/D4FO00991F
Jun Zhang , Qingmei Liu , Fudie Gu , Yan Li , Huiying Chen , Meng Liu , Yu Zhou , Hong Liu , Xiaofeng Wei and Guangming Liu
Abstract
In order to explore the in vivo anti-food allergy activity of Lactobacillus.sakei subsp.sakei-fermented Eucheuma spinosum polysaccharides F1-ESP-3 (Earlier reports). The OVA-induced food allergy mouse model was established by ascites immunization and gavage. The weight, temperature, incidence of diarrhea, levels of allergic mediators and inflammatory factors in serum of mice were analyzed. We analyzed the differentiation of mice spleen lymphocytes and the proportion of sensitized mast cells by Flow Cytometry. The intestinal barrier status of mice was analyzed by intestinal pathological tissue sections and microbiota sequencing. The results showed that F1-ESP-3 could alleviate the food allergy symptoms of mice, such as hypothermia and loose stool; Levels of OVA specific immunoglobulin E, mast cell protease and histamine in the serum of sensitized mice and the proportion of dendritic cells and mast cells in mouse spleen were significantly reduced; In addition, F1-ESP-3 can protect the intestinal barrier and further improve the intestinal microenvironment of food allergic mice by regulating the abundance of Bacteroidetes and Firmicutes. F1-ESP-3 can further improve the intestinal microenvironment of food allergic mice by up regulating the levels of Lachnospiraceae, and may affecting the signal pathways such as NOD like receptor, MAPK, I kappa B and antigen processing and presentation.
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