Thesis by Aukelise Charlotte Madeleine Theodora de Wolf
Chapter 1. General introduction
Part I. Monitoring the effects of preventive immunomodulatory therapies on regulatory T cells
Chapter 2. Regulatory T cell frequencies and phenotypes following antiviral vaccination
Part II. Monitoring the effects of non-cell-based medicinal products on regulatory T cells
Chapter 3. An arthritis-suppressive and Treg cell-inducing CD4+ T cell epitope is functional in the context of HLA-restricted T cell responses
Chapter 4. Development of an in vitro assay to test B29 peptide responsiveness in peripheral blood samples
Chapter 5. Dawn of monitoring regulatory T cells in (non-)clinical studies: their relevance is slowly recognised
Part III. Regulating in vitro functionality testing of cell-based medicinal products
Chapter 6. Regulatory perspective on in vitro potency assays for human mesenchymal stromal cells used in immunotherapy
Chapter 7. Regulatory perspective on in vitro potency assays for human T cells used in anti-tumour immunotherapy
Chapter 8. Regulatory perspective on in vitro potency assays for human dendritic cells used in anti-tumour immunotherapy
Chapter 9. Summarising discussion
Chapter 10. Addendum
© 2018 Charlotte de Wolf
All rights reserved. Published manuscripts and figures were reprinted in this thesis with permission from the publishers. No part of this thesis may be reproduced or transmitted in any form or by any means, electronical or mechanical, without prior permission of the author.
The research described in this thesis was carried out at:
– the Department of Infectious Diseases and Immunology, Division of Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
– the Department of Quality, Division of Biological Medicinal Products, Medicines Evaluation Board, Utrecht, The Netherlands. The research described in Chapter 2 was performed within the framework of the European Union’s Seventh Framework Programme BioVacSafe.