Welcome to the research repository of Mastopedia.com: your home on the web for all things mast cell related. Whether you’re seeking a diagnosis, you’re a newly diagnosed patient, a doctor or researcher, if you are seeking to connect or to uncover the latest information on Mastocytosis or Mast Cell Disorders, you’re in the right place.

If you find a particular research excerpt interesting, and want to follow the reference links, you may be sent to a database where you need to purchase a log in.  See “about” for tips on how you might obtain a copy of the research at no charge to you.

Get back to the main Mastopedia page at: http://www.mastopedia.com

Posted in Uncategorized | Leave a comment

Cardiac Mast Cells: Implications for Heart Failure


Journal of the American College of Cardiology, Volume 49, Issue 10, 13 March 2007, Pages 1107

Ulrich W. Kolck, MD, Kirsten Alfter, MD, Jürgen Homann, MD, Ivar von Kügelgen, MD, Gerhard J. Molderings, MD

Kim et al. (1) reported that in patients with chronic heart failure (CHF), the H2-histamine receptor antagonist famotidine improved both cardiac symptoms and ventricular remodeling, which was suggested to be an indirect hint for a role of histamine and its receptors in the failing heart. More direct conclusions might be deduced from investigations of patients with systemic mastocytosis who have an increased myocardial histamine concentration due to an increased mast cell density in the heart. Here we report findings observed in 17 patients with systemic mast cell activation disorder (2) who, among other symptoms, had a mast cell mediator-induced tachycardia indicating a significant infiltration in the heart by pathologically activated mast cells. One should expect that, because of the long time course of the disease (median duration of illness: 14 years), a contribution of a continuously increased histamine concentration in the heart should have become observable in those patients. In echocardiography, left ventricular diastolic and systolic diameter, fractional shortening, and ejection fraction were not pathologically altered in our patients. However, a diastolic left ventricular dysfunction occurred in 12 of 17 patients as determined by pulse wave- and/or tissue-Doppler imaging, which represents a sensitive first sign of a myocardial textural alteration. In 5 of those 12 patients a left ventricular hypertrophy was observed. Because of the absence of other precipitating factors, these alterations are probably due to the remodeling effect of prohypertrophic cytokines and proteases and profibrotic growth factors synthesized and secreted by mast cells (3).

In conclusion, our findings do not support the contention that an increased myocardial histamine concentration alone, even over a long period, leads to CHF because of a direct effect on the cardiac myocytes. The beneficial effect of the H2-histamine receptor antagonist famotidine on CHF described by Kim et al. (1) may rather indicate an indirect role of histamine in the evolution and progression of CHF. In this context, it is conceivable that H2-histamine receptor antagonists may reduce the activity of the mast cells in the heart by blocking H2-histamine receptors on them and, thereby, may reduce release of those mast cell mediators which, according to animal experiments and to our aforementioned findings, can induce a remodeling of the heart with an associated functional distortion.

Posted in Uncategorized | Leave a comment

Safety and efficacy of pregabalin in adolescents with fibromyalgia: a randomized, double-blind, placebo-controlled trial and a 6-month open-label extension study


Pediatric Rheumatology201614:46

Lesley M. Arnold, Kenneth N. Schikler, Lucinda Bateman, Tahira Khan, Lynne Pauer, Pritha Bhadra-Brown, Andrew Clair, Marci L. Chew, Joseph Scavone and on behalf of the Pregabalin Adolescent Fibromyalgia Study Group



Fibromyalgia (FM) is a common pain condition characterized by widespread musculoskeletal pain and tenderness. Pregabalin is an approved treatment for adults in the United States, but there are no approved treatments for adolescents with FM.


This was a 15-week, randomized, double-blind, placebo-controlled study and 6-month open-label safety trial of flexible-dose pregabalin (75–450 mg/day) for the treatment of adolescents (12–17 years) with FM. Primary outcome was change in mean pain score at endpoint (scored from 0–10, with 24-h recall). Secondary outcomes included global assessments and measures of pain, sleep, and FM impact.


A total of 107 subjects were randomized to treatment (54 pregabalin, 53 placebo) and 80 completed the study (44 pregabalin, 36 placebo). Improvement in mean pain score at endpoint with pregabalin versus placebo was not statistically significant, treatment difference (95 % CI), −0.66 (−1.51, 0.18), P = 0.121. There were significant improvements with pregabalin versus placebo in secondary outcomes of change in pain score by week (P < 0.05 for 10 of 15 weeks); change in pain score at week 15 (1-week recall), treatment difference (95 % CI), −0.87 (−1.68, −0.05), P = 0.037; and patient global impression of change, 53.1 % versus 29.5 % very much or much improved (P = 0.013). Trends toward improvement with pregabalin in other secondary outcomes measuring pain, sleep, and FM impact were not significant. Safety was consistent with the known profile of pregabalin in adults with FM.


Pregabalin did not significantly improve the mean pain score in adolescents with FM. There were significant improvements in secondary outcomes measuring pain and impression of change.

Trial registrations

NCT01020474; NCT01020526.


Juvenile fibromyalgia Clinical trial Pain Pregabalin

© The Author(s). 2016

Posted in Uncategorized | Leave a comment

Insect Sting Reactions and Specific IgE to Venom and Major Allergens in a General Population


Int Arch Allergy Immunol 2016;170:194-200

Mosbech H.,· Tang L., Linneberg A. (Allergy Clinic, Copenhagen University Hospital Gentofte, Hellerup, Research Centre for Prevention and Health, and Department of Clinical Experimental Research, Rigshospitalet, Glostrup, and Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark)


Background: Insect sting reactions are frequently reported, but population studies documenting the frequency and the relation to IgE-sensitization and serum tryptase are scarce. Methods: Questionnaire data and results from measurements of specific IgE against venom, major allergens and cross-reacting carbohydrate determinants (CCDs) were collected from 2,090 adult participants in a cross-sectional survey. Results: 13% of the population reported symptoms of sting reactions and about half were systemic in nature. In all, 15% were sensitized to venom but only 31% of these had reacted to stings and only 38% of those with reactions had IgE to venom. In addition, 12% with IgE to venom were double-sensitized (DS), i.e. to both bee and wasp venom. Among DS IgE to major venom allergens, rApi m 1, rVes v 1 and rVes v 5 were negative and of no help in 31%, but 59% could be identified as likely sensitized to bee or wasp. IgE to CCDs occurred in only 0.7%, but 80% of these were DS. Finally, 36% with IgE to CCDs had had symptoms, mostly local. Serum tryptase was not associated with a history of sting reactions. Conclusions: In a temperate climate, self-reported insect sting reactions and sensitization to venom are frequent, but in most cases, these are not seen in the same individual. In DS individuals, measurements of IgE to major allergens can be helpful in some but not all cases and additional analyses are needed. IgE to CCDs may have some clinical relevance.

© 2016 S. Karger AG, Basel

Posted in Uncategorized | Leave a comment

Study of Mast cells in non neoplastic skin lesions – study on 72 cases


Study of Mast cells in non neoplastic skin lesions – study on 72 cases

Prashanth Kumar M (Associate Professor, Department of Pathology, Prathima Institute of Medical Sciences, Karimnagar, Telangana)



Skin, the largest external organ of human body, is associated with a gamut of lesions. Clinical manifestations include erythema, itching, edema, swelling, vesicles, bullae, alterations in pigmentation etc. Mast cell is an integral cellular component of skin and is believed to respond to diverse range of stimuli. Mast cells can regulate tissue changes that confer either a beneficial or a harmful effect depending upon specific circumstances. In the present study, an attempt is made to evaluate mast cell profile (density) in various non neoplastic skin lesions. No attempt is made to assess their role in different cutaneous disorders.

Materials & Methods:

A prospective analytical histopathological study of non-neoplastic skin lesions was carried out during June 2004 –May 2005. A total of seventy two cases were included in this study.


Irrespective of category, lesions of bullous mastocytosis showed highest mast cell density and the lowest numbers were seen in scleroderma.


Majority of inflammatory lesions, infective and non-infective, showed definite changes in mast cell numbers and distribution patterns (like perivascular and interstitial) when compared to normal skin controls. Lesions with epidermal involvement showed increased number of mast cells in superficial dermis and lesions with dermal involvement showed dermal predominance of mast cells.


Mast cells, non neoplastic skin lesions, lichen planus, psoriasis

Posted in Uncategorized | Leave a comment

Neuropeptide-Induced Mast Cell Degranulation and Characterization of Signaling Modulation in Response to IgE Conditioning


ACS Chem. Biol., Article ASAP – Publication Date (Web): August 31, 2016

Benjamin M. Manning, Sarah M. Gruba, Audrey F. Meyer, and Christy L. Haynes (Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, United States)


As tissue-resident immune cells, mast cells are frequently found in close proximity to afferent neurons and are subjected to immunoactive mediators secreted by these neurons, including substance P (SP) and calcitonin gene-related peptide (CGRP). Neurogenic inflammation is thought to play an important role in the pathophysiology of many diseases. Unraveling the cellular mechanisms at the interface between the immune response and the peripheral nervous system is important for understanding how these diseases arise and progress. In this work, mast cell degranulation following direct exposure to CGRP and SP was studied both at the bulk and single-cell levels to characterize the mouse peritoneal mast cell response to neuropeptides and compare this response to well-studied mast cell activation pathways. Results show that mast cells secrete fewer chemical messenger-filled granules with increased IgE preincubation concentrations. The biophysical characteristics of mast cell degranulation in response to SP and CGRP is in many ways similar to calcium ionophore-induced mast cell degranulation; however, neuropeptide-stimulated mast cells secrete reduced chemical messenger content per secretion event, resulting in an overall relative decrease in secreted chemical messengers.

Copyright © 2016 American Chemical Society

Posted in Uncategorized | Leave a comment

Mast cell – glia dialogue in chronic pain and neuropathic pain: blood-brain barrier implications


CNS Neurol Disord Drug Targets. 2016 Aug 29. [Epub ahead of print]

Skaper SD (Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy)


Mast cells and microglia, working singly and in partnership, elaborate pro-inflammatory molecules which play key roles in a wide array of nervous system disorders. Such neuroinflammatory settings may compromise integrity of both the blood-nerve barrier and blood-brain barrier (BBB) and blood-spinal cord barrier. While both belong to the innate immune system mast cells are far more ubiquitous, are resident in peripheral nerves and the central nervous system, and can influence blood-nerve barrier characteristics. Mast cells, being near the perivasculature especially within the dura, on the brain side of the BBB, are strategically located to play havoc with the BBB. Mast cells and glia possess endogenous homeostatic mechanisms/molecules which are up-regulated following tissue damage. Such molecules include the N-acylethanolamine family. In particular, N-palmitoylethanolamine is proposed to have a key role in maintaining cellular homeostasis against external stressors provoking, for example, inflammation. This review will provide an overview relating to the pathobiology of neuroinflammation in the context of mast cells and microglia, their role in BBB integrity, and therapeutic perspectives in targeting these cells to preserve BBB function.

Posted in Uncategorized | Leave a comment

Assessment of the anti-allergenic effects of Scoparia dulcis in asthma management


African Journal of Pharmacy and Pharmacology, Vol. 10(31), pp. 638-644, 22 August, 2016

Jones Ofori-Amoah, George Asumeng Koffuor, Emmanuel Akomanin Asiamah, Hope Yao Agbemenyah and Albert Kumi Awuku (Department of Pharmacology, School of Medicine, University of Health and Allied Sciences, UHAS, Ho, Ghana; Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, KNUS Kumasi, Ghana; Department of Pathology, Komfo Anokye Teaching Hospital, Kumasi, Ghana; Department of Anatomy and Physiology, School of Medicine, University of Health and Allied Sciences, UHAS, Ho, Ghana; Department of Medical Laboratory Technology, School of Physical Sciences, University of Cape-Coast, Cape-Coast, Ghana)

Anti-allergies, including mast cell stabilizers, are used together with other anti-inflammatory drugs in the management of asthma, so as to prevent the release of pre-formed inflammatory mediators upon allergen exposure. This study was aimed at investigating the mast cell stabilizing and anti-anaphylactic effects of a 70% hydro-ethanolic extract of Scoparia dulcis (SDE) in murine models upon exposure to a known allergen. In vitro cytological and histological studies were conducted on guinea-pigs peritoneal cells and mesenteric tissues, respectively, to establish mast cell stabilization effect of the extract on compound 48/80-induced mast cell degranulation. The ability of SDE to protect mice against anaphylactic shock induced by compound 48/80 was also assessed. Preliminary phytochemical analysis was conducted on the extract using standard phyto-analytic procedures. Phytochemical screening showed the presence of tannins, alkaloids, glycosides, saponins, steroids and phenolic compounds. SDE showed significant inhibition (P < 0.001) against compound 48/80-induced degranulation in both the peritoneal and mesenteric mast cells of guinea-pigs (comparable to sodium cromoglycate and ketotifen fumarate). SDE also delayed the onset of symptoms of anaphylaxis in mice induced with compound 48/80, as well as reduced their mortality rate. The hydro-ethanolic extract of S. dulcis has significant mast cell stabilizing and anti-anaphylactic activities; making it a better adjunct in asthma management.

Key words: Anti-allergenic, Mast cell stabilization, Anti-anaphylactic, Compound 48/80, Scoparia dulcis.

Copyright © 2016

Posted in Uncategorized | Leave a comment