https://www.sciencedirect.com/science/article/abs/pii/S095656632030631X
Biosensors and Bioelectronics, Available online 19 September 2020, 112641
R.L. Espinosa, M. Garrido-Arandia, A. Romero-Sahagun, P. Herreros, L. Tramarin, M.F. Laguna, A. Díaz-Perales, M. Holgado (Universidad Politécnica de Madrid)
Highlights
• In this scientific work we report a novel assay framework inspired by the chemical and optical properties of SiO2 NPs and by the flexibility of the interferometric Fabry-Perot transducer used.
• The novelty of the methodology lies on the use of a given size of SiO2 NPs as filtering and signal amplification system to separate IgE from IgG and to improve the interferometric optical signal, respectively.
• We solved the unspecific adsorption problem in diagnostic test based on specific IgE detection by separating the objective molecule, IgE, from the rest of the serum out of the sensor.
• We developed a high performance in vitro detection system for multiplex serologic IgE which improves the specificity and selectivity of our biosensors, reducing the cross-reactivity and the matrix-effect in real serum assays.
• We demonstrated the effectivity of the system for one molecular allergen, with promising results to become a real alternative to existing CRD diagnostic tests based on specific IgE detection.
Abstract
Food allergens cause worldwide chronic diseases with a great impact on public health. Immunoglobulins E (IgEs) trigger allergic reactions by specifically binding the allergens to which the allergic patients are sensitized. In this scientific work we report for the first time a new optical interferometric in vitro system for the detection of specific IgEs (sIgEs) to the principal peach allergen (Pru p 3) in real serum samples. Interferometric Optical Detection Method (IODM) was employed for reading out the signal of Fabry-Perot based interferometers acting as biotransducers. Pru p 3 was immobilized as bioreceptor onto the sensing surface for detecting the target biomolecules, sIgEs to Pru p 3. Moreover, the demanding low concentration of IgE, compared to other analytes in real serum samples, made it necessary to use nanoparticles (NPs) for two reasons: to collect only the IgEs from the serum sample and to enhance the optical interferometric read-out signal. The methodology was validated in advance by Scanning Electron Microscopy (SEM). Consequently, we report in this article a novel high-performance in vitro detection method to recognize sIgE to molecular allergens by means of silicon dioxide (SiO2) NPs. Finally, this scientific work provides the basis for the in vitro component resolved diagnosis (CRD) of sIgEs to molecular allergens.
Keywords: Food allergy IgE detection Optical biosensors Interferometry SiO2 nanoparticles Molecular allergen Component resolved diagnosis
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